Composite

Part:BBa_K625001:Design

Designed by: Michael Eichenberger   Group: iGEM11_ETH_Zurich   (2011-09-21)


pTet - LacIM1 - Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As we want a multiplasmid system with LacI behind a Tet and a lambda promoter, we used the codon modified LacIM1 from (Basu et al, 2005) to prevent recombination between the two.


Source

Synthesized by GeneArt. Cloned into a BioBrick backbone.

References